Coding

Part:BBa_K415502:Design

Designed by: Laura Deming, Joy Jiao, Adrian Slusarczyk   Group: iGEM10_MIT   (2010-10-27)


SMAD5 L1L2 MammoBlock Entry Vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 453
    Illegal EcoRI site found at 1422
    Illegal SpeI site found at 48
    Illegal SpeI site found at 891
    Illegal PstI site found at 243
    Illegal PstI site found at 1148
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 453
    Illegal EcoRI site found at 1422
    Illegal NheI site found at 609
    Illegal SpeI site found at 48
    Illegal SpeI site found at 891
    Illegal PstI site found at 243
    Illegal PstI site found at 1148
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 453
    Illegal EcoRI site found at 1422
    Illegal BglII site found at 225
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 453
    Illegal EcoRI site found at 1422
    Illegal SpeI site found at 48
    Illegal SpeI site found at 891
    Illegal PstI site found at 243
    Illegal PstI site found at 1148
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 453
    Illegal EcoRI site found at 1422
    Illegal SpeI site found at 48
    Illegal SpeI site found at 891
    Illegal PstI site found at 243
    Illegal PstI site found at 1148
    Illegal AgeI site found at 5
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 813


Design Notes

We PCR'ed insert DNA to add attB sites to either side, then used a BP recombination to create the L1L2 Entry Vector


Source

Human SMAD5 cDNA was obtained from OpenBiosystems.

References